Translational_Unit

Part:BBa_K1500009

Designed by: Rivka Baker Keusch   Group: iGEM14_UChicago   (2014-10-17)


IPTG--GFP--DnaE

Contains an IPTG-sensitive promoter (BBa_J04500), with RBS.3 (BBa_00032), and reporter GFP (E0040) downstream, along with another RBS and the dominant negative form of the DnaE mutator.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2951
    Illegal XhoI site found at 1474
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 2040
    Illegal AgeI site found at 2994
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 870
    Illegal SapI.rc site found at 1873
    Illegal SapI.rc site found at 3757


[edit]
Categories
Parameters
None